KMID : 1234420110390010049
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Korean Journal of Microbiololgy and Biotechnology 2011 Volume.39 No. 1 p.49 ~ p.55
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Kim Hyun-su
Lee Jae-Yeon Hwang Kyo-Yeol Cho Yong-Seok Park Young-Shik Kang Kyung-Don Seong Su-Il
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Abstract
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Thirty Streptomyces sp. and 200 Bacillus sp. isolated from Korean soils and traditional foods were screened for their abilities to inhibit ¥á-glucosidase and produce 1-deoxynojirimycin (DNJ). This screening identified a Bacillus sp. bacterium that strongly inhibited ¥á-glucosidase and produced high levels of DNJ from Chungkookjang, a Korean traditional food. The bacterium was characterized in terms of its biochemical and molecular biological properties such as sugar utilization, cellular quinone composition, cell wall fatty acid composition, and 16S rDNA sequence. In addition, scanning electron microscopy was used to visualize the morphology of the bacterium. These analyses identified the bacterium as B. subtilis, a bacterium with Generally Recognized as Safe (GRAS) status. The selected strain was named B. subtilis MORI.
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